AZENTA NGS DATA REPORT

1. Project Summary

Project ID:	30-775111958
Customer:	SeanOConnell
BI:	GENEWIZ-NGS-BI

2. Description of Workflow

2.1. Experiment Workflow

16S/18S/ITS rRNA is composed of conserved and hypervariable regions. Whereas conserved regions are not significantly different across various microbial strains, the sequences of hypervariable regions are genus or species-specific, and differ in accordance to phylogenetic difference. Therefore, 16S/18S/ITS rDNA serve as identifiers of biological species, and are important for microbial phylogeny and taxonomic identification. 16S/18S/ITS rDNA amplicon sequencing has become an important tool for the study of the composition of microbial communities in environment.

16S rDNA amplicon sequencing includes the library construction using specific primers to amplify the variable region of prokaryotic 16S rDNA and data analysis of the 16S rDNA variable region sequence to identify the composition and abundance of prokaryotic microorganisms in the environment. The proprietary workflow at Azenta effectively amplifies the three variable regions of 16S rDNA (V3, V4, V5) and accurately identifies various species including archaea. For samples with eukaryotic contamination, only V3, V4 region are amplified. 18S / ITS rDNA amplicon sequencing includes the library construction using specific primers to amplify the variable region of eukaryotic 18S / ITS rDNA and data analysis to identify the composition and abundance of eukaryotic microorganisms in the environment. Illumina MiSeq sequencing platform is widely used for 16S / 18S / ITS rDNA amplicon sequencing because of its deep sequencing depth, high throughput, short run-time and high sequencing accuracy as well as reasonable cost. In recent years, pair-end chemistry has enabled MiSeq sequencing platform to read as long as 600bp, which further increased the accuracy of the results.

16S / 18S / ITS rDNA amplicon sequencing procedure includes genomic DNA extraction, quality control, rDNA variable region amplification, library construction, high-throughput sequencing and data analysis. All the steps are important for data quality and quantity, which in turn affects the subsequent data analysis. In order to ensure data accuracy and reliability, every step has to pass strict quality control before pooling the library by adjusting the volume of each library according to the target data volume for Illumina MiSeq sequencing. The workflow is as below:

Figure 2.1 Microflora diversity experimental workflow

2.2. Bioinformatics Analysis Workflow

Figure 2.2 Microflora diversity data analysis workflow

Note:

(1) If the number of samples is less than 3, comparative analysis of diversity cannot be performed.

(2) The differential statistical analysis is meaningful only if there are at least three replicates in the biology group.

(3) Environmental factors are required for correlation analysis of community composition and environmental factors as well as for CCA / RDA analysis.

(4) Intestinal-type analysis only applies to animal or human intestinal or stool samples.

(5) Analysis with * are not included in the standard analysis and can be selected and analyzed according to individual sample and project.

3. Analysis

3.1. Sample Summary Statistics

Sample	Length(bp)	#Reads	Bases(bp)	Q20(%)	Q30(%)	GC(%)	N(ppm)
C1	241.03	70,290	16,942,091	96.58	91.10	46.15	141.19
C2-2	246.75	239,092	58,995,504	96.88	91.46	42.85	32.90
C3	248.29	241,762	60,028,275	96.92	91.55	49.62	43.16
P1	246.83	241,780	59,679,343	97.10	91.85	51.72	31.30
P2	244.71	240,710	58,903,760	96.84	91.55	52.44	28.44
P3	247.37	240,584	59,512,644	97.09	91.83	50.27	32.87

3.2. Sequencing Data Quality Optimization

Sequencing errors might occur in high-throughput sequencing, and it is common that bases toward the end of the sequence reads have lower than average quality. In order to obtain higher quality and more accurate bioinformatic analysis results, it is necessary to optimize the raw data of the sequencing to obtain higher quality and more accurate bioinformatics analysis results.

Analysis software: Cutadapt(v1.9.1), Vsearch(1.9.6), Qiime(1.9.1)

Steps and parameters for optimization:

(1) The two sequences of each read pair were merged according to overlapping sequences. The read merge is deemed to be successful only if the overlapping sequence is least 20bp long. After merging, undetermined bases (N) were removed from the resulting sequence.

(2) Primer and adapter sequences were removed. Then the 5' and 3' bases with Q score lower than 20 were also removed. The resulting sequences with length > 200bp would pass this step of processing.

(3) The sequences obtained were then aligned to UCHIME 'Gold' database to identify and remove chimera sequence. Sequences passed this filtering step are deemed as clean data ready for analysis.

Sample	#PE_reads	#Nochimera	AvgLen(bp)	GC(%)	Effective(%)
C1	35,145	30,086	326.32	45.18	85.61
C2_2	119,546	113,317	332.75	42.81	94.79
C3	120,881	115,192	339.29	49.12	95.29
P1	120,890	113,295	332.64	50.93	93.72
P2	120,355	105,574	323.82	51.36	87.72
P3	120,292	113,943	339.94	49.86	94.72

Figure 3.2 Effective sequences length distribution

3.3. OTU Analysis and Species Annotation

3.3.1. OTU clustering

OTU is an operational definition of a classification unit (genus, species, grouping, etc.) commonly used in population genetics to facilitate data analysis. In bioinformatics each sequence obtained from sequencing is assumed to be derived from a single species. All the sequences in a sample are classified to obtain information on species and genus. By classification, the sequences are grouped according to their similarity, and one group is an OTU. Typically, OTU clusters are defined by a 97% identity threshold for data statistics and analysis.

Analysis software:Qiime(1.9.1),Vsearch(1.9.6)

Analysis methods and steps:

(1) Unique sequences were extracted from the optimized sequences with the read count information.

(2) The unique sequences with 1 read count were removed.

(3) OTU clustering of unique sequences (read count > 1) was performed with similarity of 97%, and chimeric sequences were further removed to obtain the representative OTU sequences.

(4) All optimized sequences were compared with OTU representative sequences, and sequences of >97% similarity to a specific OTU representative sequence are considered to be of the same OTU. Finally, the OTU abundances were also summarized in the results table.

The following table shows the statistics of the sequence number in each sample's OTU:

OTU_ID	C1	C2-2	C3	P1	P2	P3	taxonomy
OTU4	17,966	10,006	175	329	231	1	k__Fungi;p__Mucoromycota;c__Mucoromycetes;o__Mucorales;f__Mucoraceae;g__Mucor;s__Mucor_circinelloides
OTU5	45	9,258	161	1	2	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Tricholomataceae;g__Pseudotricholoma;s__Pseudotricholoma_umbrosum
OTU7	0	0	1,187	21	32	8,029	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula
OTU8	214	82	532	3,803	2,935	1,550	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Tremellales;f__Trimorphomycetaceae;g__Saitozyma;s__Saitozyma_podzolica
OTU6	0	0	5	3,105	2,305	1,745	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU9	63	87	1,536	3,085	467	25	k__Fungi;p__Basidiomycota;c__Geminibasidiomycetes;o__Geminibasidiales;f__Geminibasidiaceae;g__Geminibasidium;s__unidentified
OTU14	0	0	1	1,127	2,425	6	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae
OTU11	1,058	311	1,682	254	335	360	k__Fungi;p__Ascomycota
OTU12	27	24	195	205	1,568	1,310	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Leotiomycetes_fam_Incertae_sedis;g__Leohumicola;s__Leohumicola_verrucosa
OTU10	0	0	3	2,709	97	296	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Lactarius
OTU15	0	0	1	2,148	818	7	k__Fungi;p__Ascomycota
OTU16	16	767	1,681	3	66	401	k__Fungi;p__Ascomycota
OTU13	19	32	111	477	1,591	754	k__Fungi;p__Ascomycota;c__Leotiomycetes
OTU28	0	0	2,642	7	12	6	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Boletaceae;g__unidentified;s__unidentified
OTU19	0	0	2	12	6	2,364	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_crustosa
OTU31	54	489	1,673	100	24	2	k__Plantae;p__unidentified;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU22	0	0	17	7	2,452	3	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Sclerodermataceae;g__Scleroderma;s__Scleroderma_citrinum
OTU17	19	0	9	3	2,317	6	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Trichocomaceae;g__Sagenomella;s__Sagenomella_verticillata
OTU18	0	0	3	2,051	2	2	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Elaphomycetaceae;g__Elaphomyces;s__Elaphomyces_maculatus
OTU23	7	0	91	170	1,192	668	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Trichocomaceae;g__Sagenomella;s__Sagenomella_striatispora
OTU44	0	0	47	414	505	948	k__Fungi;p__Ascomycota
OTU26	10	1,499	372	2	3	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU24	0	0	0	1	1,946	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_cyanoxantha
OTU25	0	0	2	3	254	1,512	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU30	0	0	0	886	864	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_melzeri
OTU27	12	1,580	29	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU20	0	0	4	1,097	346	130	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Astraeaceae;g__Astraeus;s__Astraeus_hygrometricus
OTU39	0	0	2	820	3	410	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU42	749	108	130	195	273	365	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Leotiomycetes_fam_Incertae_sedis;g__Leohumicola;s__unidentified
OTU33	0	0	1,062	2	5	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Hygrophoraceae
OTU32	31	2	121	147	79	735	k__Fungi;p__Ascomycota
OTU29	3,600	2	1	0	1	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Elaphomycetaceae;g__Elaphomyces;s__unidentified
OTU35	4	940	0	0	0	4	k__Fungi;p__Ascomycota;c__Geoglossomycetes;o__Geoglossales;f__Geoglossaceae;g__Trichoglossum;s__Trichoglossum_walteri
OTU36	3	0	808	4	1	1	k__Fungi;p__Basidiomycota
OTU41	2	0	270	108	435	35	k__Fungi;p__Mucoromycota;c__Endogonomycetes;o__GS20;f__unidentified;g__unidentified;s__unidentified
OTU40	0	0	1	76	654	112	k__Fungi;p__Ascomycota
OTU2	1,334	464	0	4	3	2	k__Fungi
OTU52	1	176	470	0	2	2	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula
OTU64	0	0	3	213	102	232	k__Fungi;p__Ascomycota
OTU48	3	0	572	2	5	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavulinopsis;s__Clavulinopsis_laeticolor
OTU47	1	1	36	337	87	97	k__Fungi;p__Ascomycota;c__Saccharomycetes;o__Saccharomycetales;f__unidentified;g__unidentified;s__unidentified
OTU109	2	0	300	57	63	121	k__Fungi;p__Ascomycota
OTU45	0	0	1	2	293	236	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae;g__Pachyphloeus;s__unidentified
OTU49	0	0	0	2	1	457	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae
OTU58	0	0	446	1	2	3	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_rubellipes
OTU77	28	69	90	64	152	81	k__Fungi;p__Ascomycota;c__Leotiomycetes
OTU53	4	0	463	2	2	0	k__Fungi;p__Ascomycota
OTU54	0	0	0	246	243	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Inocybaceae;g__Inocybe;s__Inocybe_calamistrata
OTU73	0	0	405	0	2	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae;g__Hydnobolites
OTU487	4	225	2	0	30	166	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Leotiomycetes_fam_Incertae_sedis;g__Leohumicola;s__Leohumicola_verrucosa
OTU60	0	0	124	106	44	154	k__Fungi;p__Basidiomycota;c__Pucciniomycotina_cls_Incertae_sedis;o__GS25;f__unidentified;g__unidentified;s__unidentified
OTU43	0	0	0	261	72	73	k__Fungi;p__Ascomycota
OTU74	0	0	0	2	2	387	k__Fungi;p__Ascomycota
OTU70	0	0	11	94	246	51	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU82	11	3	34	54	57	207	k__Fungi;p__Ascomycota
OTU51	0	13	188	99	55	39	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU72	2	373	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula
OTU62	0	0	341	1	2	0	k__Fungi;p__Ascomycota
OTU57	0	7	344	1	1	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Tuberaceae;g__Tuber
OTU56	1	2	239	59	13	16	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU61	0	0	0	330	4	3	k__Plantae
OTU1	624	173	0	0	0	0	k__Fungi
OTU71	7	6	123	51	81	81	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU66	1	308	0	0	0	0	k__Fungi;p__Ascomycota
OTU63	6	17	179	18	50	39	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU149	0	0	0	172	0	171	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Boletaceae;g__Tylopilus;s__Tylopilus_atronicotianus
OTU78	3	3	112	115	88	8	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Nectriaceae;g__Ilyonectria;s__Ilyonectria_radicicola
OTU46	9	3	13	155	135	5	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Bondarzewiaceae;g__Bondarzewia;s__Bondarzewia_mesenterica
OTU84	3	0	132	14	11	140	k__Fungi;p__Ascomycota
OTU129	125	44	220	7	1	1	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU76	0	0	226	28	28	7	k__Fungi;p__Ascomycota
OTU68	0	0	246	1	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Lactarius;s__Lactarius_chrysorrheus
OTU59	5	2	41	17	43	120	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_pseudozygospora
OTU94	0	0	0	56	3	211	k__Fungi;p__Ascomycota
OTU67	0	120	125	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Hygrophoraceae;g__Cuphophyllus;s__Cuphophyllus_lacmus
OTU106	0	0	34	2	117	115	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Chaetosphaeriales;f__Chaetosphaeriaceae;g__Chaetosphaeria;s__unidentified
OTU90	0	0	239	1	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_crustosa
OTU69	6	17	56	14	47	96	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__Umbelopsidales;f__Umbelopsidaceae;g__Umbelopsis;s__Umbelopsis_dimorpha
OTU79	0	0	157	3	17	35	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU83	25	0	32	56	30	105	k__Fungi;p__Ascomycota;c__Ascomycota_cls_Incertae_sedis;o__Ascomycota_ord_Incertae_sedis;f__Ascomycota_fam_Incertae_sedis;g__Trichocladium;s__Trichocladium_opacum
OTU150	0	3	47	28	6	142	k__Fungi;p__Ascomycota
OTU100	0	25	35	15	70	85	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae;g__Metarhizium;s__Metarhizium_carneum
OTU115	19	0	201	2	2	0	k__Fungi;p__Ascomycota
OTU96	2	7	21	36	38	104	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Pseudeurotiaceae;g__Pseudogymnoascus;s__Pseudogymnoascus_roseus
OTU122	0	0	183	0	2	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Lactarius;s__Lactarius_quietus
OTU113	57	0	8	0	190	6	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU159	1	8	81	71	37	1	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_humilis
OTU98	1	167	29	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria
OTU125	0	0	190	0	3	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Atheliales;f__Atheliaceae;g__Byssocorticium;s__Byssocorticium_pulchrum
OTU81	15	0	3	171	5	8	k__Fungi;p__Ascomycota;c__Pezizomycotina_cls_Incertae_sedis;o__GS35;f__unidentified;g__unidentified;s__unidentified
OTU92	0	24	105	27	23	3	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium;s__Penicillium_verhagenii
OTU154	451	32	7	15	0	12	k__Plantae;p__Anthophyta;c__Monocotyledonae;o__Poales;f__Poaceae;g__unidentified;s__unidentified
OTU107	2	0	9	92	11	61	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Onygenales;f__unidentified;g__unidentified;s__unidentified
OTU99	0	4	89	9	76	12	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_beljakovae
OTU104	0	0	76	45	7	27	k__Fungi;p__Ascomycota;c__Archaeorhizomycetes;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU80	0	0	169	0	2	0	k__Fungi;p__Ascomycota
OTU128	0	0	0	1	0	169	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita;s__Amanita_alboflavescens
OTU126	18	3	157	0	1	0	k__Fungi;p__Ascomycota
OTU88	2	175	0	0	0	1	k__Fungi;p__Ascomycota
OTU89	7	1	20	42	33	79	k__Fungi;p__Ascomycota
OTU97	0	0	0	1	0	148	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Nectriaceae;g__Gliocladiopsis;s__Gliocladiopsis_curvata
OTU240	0	0	135	10	10	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU91	0	0	154	1	2	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria;s__Clavaria_falcata
OTU102	23	0	143	1	2	0	k__Fungi;p__Ascomycota
OTU103	0	0	137	0	1	0	k__Fungi;p__Ascomycota;c__Xylonomycetes;o__GS34;f__unidentified;g__unidentified;s__unidentified
OTU134	0	32	9	99	0	3	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae;g__Tomentella
OTU93	0	0	145	0	0	0	k__Fungi;p__Ascomycota
OTU157	6	17	81	2	24	7	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_humilis
OTU112	0	0	0	25	1	93	k__Fungi
OTU85	0	0	1	71	56	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU191	0	0	0	39	0	68	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__unidentified
OTU138	0	0	112	0	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavulinopsis
OTU120	1	1	52	27	8	29	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Filobasidiales;f__Piskurozymaceae;g__Solicoccozyma;s__Solicoccozyma_terricola
OTU145	0	5	59	9	29	20	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_horticola
OTU105	0	1	123	0	0	0	k__Fungi;p__Ascomycota
OTU121	0	0	11	3	74	46	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium;s__unidentified
OTU3	5	0	11	32	62	18	k__Fungi
OTU133	2	113	0	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Dermateaceae
OTU142	0	0	112	3	2	0	k__Fungi;p__Ascomycota
OTU110	0	0	0	138	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiaceae;g__Cudoniella;s__Cudoniella_acicularis
OTU165	0	0	101	0	1	0	k__Fungi
OTU184	0	0	108	5	3	5	k__Fungi
OTU123	0	0	115	1	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Calostomataceae;g__Calostoma;s__Calostoma_cinnabarinum
OTU228	9	0	105	1	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU177	0	0	8	26	0	75	k__Fungi;p__Ascomycota
OTU186	0	0	9	38	40	25	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU95	0	0	0	113	0	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae
OTU173	9	9	19	38	3	39	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Sordariales_fam_Incertae_sedis;g__Dendrosporium;s__unidentified
OTU269	0	0	1	0	2	97	k__Fungi
OTU204	0	0	0	0	0	96	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae;g__Tomentella
OTU124	7	0	100	0	2	5	k__Fungi;p__Ascomycota
OTU114	365	1	10	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_chloroides
OTU152	0	0	0	1	1	101	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Lactifluus
OTU164	0	0	68	6	7	7	k__Fungi
OTU101	0	0	99	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita
OTU206	0	0	0	0	0	105	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae;g__Rhizophagus;s__Rhizophagus_irregularis
OTU181	1	10	86	0	1	0	k__Fungi;p__Ascomycota
OTU250	2	5	32	12	4	23	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiales_fam_Incertae_sedis;g__Leptodontidium;s__Leptodontidium_trabinellum
OTU135	0	1	53	2	34	14	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Pseudeurotiaceae
OTU132	1	94	0	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Leotiomycetes_fam_Incertae_sedis;g__Leohumicola;s__unidentified
OTU167	0	0	68	0	0	0	k__Fungi
OTU193	0	0	0	93	0	1	k__Fungi;p__Basidiomycota
OTU197	0	0	0	14	1	54	k__Fungi;p__Ascomycota
OTU111	0	0	0	84	4	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_subvinosa
OTU163	3	4	80	2	2	1	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_angusta
OTU148	0	0	0	40	53	5	k__Fungi;p__Ascomycota;c__Ascomycota_cls_Incertae_sedis;o__Ascomycota_ord_Incertae_sedis;f__Ascomycota_fam_Incertae_sedis;g__Pseudoclathrosphaerina;s__unidentified
OTU153	1	0	13	0	0	69	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae;g__Rhizophagus
OTU171	2	0	66	3	4	5	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium;s__unidentified
OTU189	2	14	15	18	23	10	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU211	108	51	4	0	0	0	k__Fungi;p__Mucoromycota;c__Mucoromycetes;o__Mucorales;f__Mucoraceae;g__Mucor;s__Mucor_circinelloides
OTU354	0	0	78	0	1	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU203	0	0	0	6	1	83	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU139	0	0	78	1	1	0	k__Fungi;p__Ascomycota
OTU199	0	0	0	30	0	39	k__Fungi;p__Ascomycota
OTU146	19	0	36	3	1	34	k__Fungi;p__Ascomycota
OTU221	0	3	31	28	12	1	k__Fungi;p__Ascomycota
OTU119	0	25	48	0	2	0	k__Fungi;p__Ascomycota
OTU151	0	4	0	15	40	14	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Nectriaceae
OTU118	0	35	33	1	1	0	k__Fungi;p__Ascomycota;c__Orbiliomycetes;o__Orbiliales;f__Orbiliaceae
OTU196	9	2	0	14	13	40	k__Fungi;p__Ascomycota
OTU591	1	0	65	0	2	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU108	0	0	79	0	0	1	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Chaetomiaceae
OTU233	12	3	68	0	2	0	k__Fungi;p__Ascomycota
OTU180	0	9	47	0	3	6	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU155	5	9	12	14	14	10	k__Fungi;p__Basidiomycota;c__Microbotryomycetes;o__Leucosporidiales;f__unidentified;g__unidentified;s__unidentified
OTU224	0	0	35	0	17	17	k__Fungi
OTU234	0	0	61	0	0	1	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae;g__unidentified;s__unidentified
OTU442	160	4	0	11	7	8	k__Fungi
OTU166	2	0	16	1	0	39	k__Fungi;p__Ascomycota
OTU140	0	0	31	9	16	0	k__Fungi;p__Basidiomycota
OTU273	0	32	3	0	0	31	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria;s__Clavaria_flavipes
OTU198	0	0	0	64	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Inocybaceae;g__Inocybe;s__Inocybe_cookei
OTU201	0	0	0	33	41	0	k__Fungi;p__Basidiomycota
OTU230	0	0	69	1	0	0	k__Fungi
OTU222	0	0	54	0	2	1	k__Fungi
OTU207	0	9	10	0	0	36	k__Fungi;p__GS19;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU267	0	0	0	0	0	61	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae
OTU144	0	0	59	0	1	0	k__Fungi;p__Ascomycota
OTU229	24	0	51	0	0	0	k__Fungi;p__Ascomycota
OTU719	0	0	0	0	0	62	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_crustosa
OTU175	0	0	51	0	2	0	k__Fungi
OTU141	0	0	51	1	0	1	k__Fungi;p__Ascomycota
OTU634	0	0	1	49	2	5	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Leotiomycetes_fam_Incertae_sedis;g__Leohumicola
OTU266	8	0	2	0	18	29	k__Fungi;p__Ascomycota;c__Xylonomycetes;o__GS34;f__unidentified;g__unidentified;s__unidentified
OTU143	0	0	16	0	2	32	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales
OTU131	213	0	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Tricholomataceae;g__Tricholoma;s__Tricholoma_columbetta
OTU223	0	0	56	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU237	0	0	45	0	0	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pyronemataceae;g__Genea;s__Genea_hispidula
OTU174	0	0	56	1	1	2	k__Fungi
OTU183	0	0	45	0	3	0	k__Fungi;p__Basidiomycota;c__Geminibasidiomycetes;o__Geminibasidiales;f__Geminibasidiaceae;g__Geminibasidium;s__unidentified
OTU227	0	0	64	0	2	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae;g__Pseudotomentella;s__unidentified
OTU216	1	14	30	0	1	0	k__Fungi;p__Ascomycota
OTU162	12	2	14	6	1	11	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Polyporales;f__Ganodermataceae
OTU136	0	2	37	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_variata
OTU185	0	1	48	7	1	0	k__Fungi;p__Ascomycota
OTU161	0	26	13	0	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_gemmifera
OTU225	0	0	48	0	0	0	k__Fungi;p__Ascomycota
OTU318	0	0	42	6	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria;s__unidentified
OTU246	0	0	37	1	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria;s__Clavaria_fragilis
OTU412	1	0	0	0	41	6	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__Umbelopsidales;f__Umbelopsidaceae;g__Umbelopsis;s__Umbelopsis_ramanniana
OTU305	0	7	40	1	0	0	k__Plantae;p__unidentified;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU361	4	3	14	30	0	2	k__Fungi;p__Ascomycota
OTU158	0	31	12	0	0	1	k__Fungi;p__Ascomycota
OTU314	6	0	41	0	1	0	k__Fungi;p__Ascomycota
OTU178	0	0	46	0	0	0	k__Fungi;p__Ascomycota;c__Orbiliomycetes;o__Orbiliales;f__Orbiliaceae
OTU253	0	5	0	28	11	11	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales
OTU200	0	0	0	27	12	12	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Chaetomiaceae;g__Chaetomium;s__Chaetomium_homopilatum
OTU192	0	0	0	29	2	9	k__Fungi;p__Ascomycota
OTU271	0	0	0	0	0	47	k__Fungi;p__Ascomycota
OTU248	0	0	54	0	0	1	k__Fungi;p__Ascomycota
OTU385	1	0	0	12	1	20	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Dermateaceae
OTU380	0	0	5	13	12	8	k__Fungi;p__Ascomycota
OTU241	1	0	42	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Cortinariaceae;g__Cortinarius
OTU170	0	0	38	1	1	0	k__Fungi;p__Ascomycota
OTU251	0	0	0	42	0	0	k__Fungi;p__Ascomycota
OTU623	0	0	13	32	2	3	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU272	0	0	0	0	1	51	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Chaetosphaeriales;f__Chaetosphaeriaceae;g__Chaetosphaeria
OTU194	0	0	0	44	0	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Elaphomycetaceae;g__Elaphomyces;s__Elaphomyces_decipiens
OTU262	0	0	0	0	1	43	k__Fungi;p__Ascomycota
OTU315	4	0	39	0	0	0	k__Fungi;p__Ascomycota
OTU137	0	0	41	0	1	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU127	0	0	0	18	1	21	k__Fungi
OTU238	0	0	40	0	3	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU182	0	0	39	0	1	0	k__Fungi;p__Ascomycota
OTU168	0	0	39	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula
OTU464	50	8	1	8	2	3	k__Fungi
OTU306	0	0	25	0	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Dissophora;s__Dissophora_ornata
OTU393	0	0	0	0	48	0	k__Fungi
OTU263	0	0	0	0	0	35	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU188	0	0	2	22	4	6	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiaceae
OTU372	0	0	0	28	7	2	k__Fungi;p__Ascomycota
OTU258	0	0	0	0	31	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Cantharellales;f__Ceratobasidiaceae;g__Ceratobasidium;s__unidentified
OTU413	0	0	0	1	11	23	k__Fungi;p__Ascomycota
OTU301	9	7	21	0	1	0	k__Fungi;p__Ascomycota
OTU244	0	0	33	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Camarophyllopsis;s__Camarophyllopsis_atrovelutina
OTU290	0	9	16	1	0	0	k__Fungi;p__Glomeromycota
OTU432	0	0	0	0	0	35	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU332	0	1	40	0	0	0	k__Fungi;p__Chytridiomycota;c__Rhizophydiomycetes;o__Rhizophydiales
OTU275	71	1	5	0	0	15	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU218	0	4	18	7	1	2	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae
OTU231	0	1	33	0	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_parvispora
OTU242	0	0	36	0	0	0	k__Fungi;p__Ascomycota
OTU295	0	18	14	0	3	0	k__Fungi;p__Ascomycota
OTU293	0	6	26	0	0	0	k__Fungi;p__Mucoromycota;c__Endogonomycetes;o__GS22;f__unidentified;g__unidentified;s__unidentified
OTU492	0	5	0	1	3	19	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Leotiomycetes_ord_Incertae_sedis;f__Leotiomycetes_fam_Incertae_sedis;g__Leohumicola;s__unidentified
OTU339	0	0	42	1	0	0	k__Fungi;p__Ascomycota
OTU300	2	1	0	0	27	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium;s__Penicillium_bialowiezense
OTU360	0	1	24	8	3	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Chaetomiaceae
OTU147	0	0	24	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Lyophyllaceae;g__Asterophora;s__Asterophora_parasitica
OTU333	0	0	21	0	0	10	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae
OTU205	0	0	0	1	0	25	k__Fungi;p__Ascomycota
OTU317	0	0	28	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU308	0	0	30	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Entolomataceae;g__Entoloma
OTU382	0	0	0	2	11	10	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Bionectriaceae;g__Clonostachys;s__Clonostachys_rosea
OTU336	0	0	26	0	0	5	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Lycoperdaceae;g__Lycoperdon
OTU311	0	0	26	0	0	0	k__Fungi;p__Ascomycota
OTU322	0	0	22	0	0	0	k__Fungi;p__Ascomycota
OTU169	1	0	21	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Chaetomiaceae
OTU187	0	0	18	0	16	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae;g__unidentified;s__unidentified
OTU417	2	0	0	0	31	1	k__Fungi;p__Ascomycota
OTU321	0	0	20	0	1	0	k__Fungi
OTU626	0	0	0	24	0	1	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales
OTU337	4	0	36	0	0	0	k__Fungi
OTU423	0	0	26	0	2	7	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU209	40	0	3	1	4	4	k__Fungi;p__Ascomycota;c__Eurotiomycetes
OTU400	0	0	0	0	6	19	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Cephalothecaceae;g__unidentified;s__unidentified
OTU239	0	0	22	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Hygrophoraceae
OTU362	9	0	0	24	3	1	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU172	1	1	20	0	0	0	k__Fungi;p__Ascomycota
OTU307	0	0	27	0	0	0	k__Fungi
OTU176	0	0	25	0	1	0	k__Fungi;p__Basidiomycota;c__Microbotryomycetes
OTU247	0	0	21	1	0	0	k__Fungi;p__Ascomycota
OTU528	0	0	14	14	0	2	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU445	4	23	0	0	0	0	k__Fungi
OTU303	0	0	15	0	0	10	k__Fungi;p__Ascomycota
OTU377	0	0	0	2	0	24	k__Fungi
OTU294	1	27	3	0	0	0	k__Fungi;p__Ascomycota
OTU390	0	0	0	0	27	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae
OTU256	0	0	2	16	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Nectriaceae;g__Volutella;s__unidentified
OTU179	0	0	17	0	0	0	k__Fungi
OTU215	0	21	11	0	0	0	k__Fungi;p__Basidiomycota;c__Geminibasidiomycetes;o__Geminibasidiales;f__Geminibasidiaceae;g__Geminibasidium;s__unidentified
OTU274	0	0	0	1	2	18	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__unidentified
OTU330	0	0	19	0	0	0	k__Fungi
OTU387	0	3	1	3	10	5	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU576	0	0	24	0	0	0	k__Fungi
OTU347	0	0	23	0	0	0	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae
OTU394	0	0	0	5	10	2	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU374	0	0	0	21	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes
OTU276	28	13	4	0	2	0	k__Fungi;p__GS19;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU403	0	0	0	0	23	0	k__Fungi;p__Ascomycota
OTU357	0	0	20	0	1	0	k__Fungi
OTU558	0	0	22	1	1	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU495	0	3	0	13	2	3	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae
OTU514	1	3	8	0	13	3	k__Fungi;p__Ascomycota;c__Sordariomycetes
OTU446	25	6	4	0	0	1	k__Fungi
OTU264	3	0	3	0	23	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Tricholomataceae;g__Delicatula;s__Delicatula_integrella
OTU370	0	0	0	23	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Nectriaceae;g__Nectria;s__Nectria_ramulariae
OTU441	46	2	0	3	1	2	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae;g__Neoascochyta;s__Neoascochyta_exitialis
OTU396	0	0	0	0	20	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Diaporthales;f__Valsaceae
OTU313	0	1	17	2	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Venturiales
OTU530	0	0	19	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Ramariopsis;s__unidentified
OTU319	0	0	17	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Cortinariaceae;g__Cortinarius
OTU325	0	0	17	0	0	0	k__Fungi;p__Ascomycota
OTU340	0	0	14	0	1	0	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae
OTU697	0	0	0	0	21	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes
OTU335	0	0	15	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Hygrophoraceae;g__Gliophorus;s__unidentified
OTU243	2	0	21	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita;s__Amanita_constricta
OTU405	0	0	0	0	1	15	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales
OTU220	3	4	14	0	0	0	k__Fungi;p__Ascomycota
OTU282	21	10	1	2	3	4	k__Fungi
OTU289	5	3	1	0	14	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium;s__unidentified
OTU404	0	0	0	0	32	0	k__Fungi;p__Ascomycota
OTU436	0	0	0	0	5	13	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae;g__Archaeospora;s__unidentified
OTU368	0	0	0	11	4	6	k__Fungi;p__Ascomycota
OTU414	0	0	0	0	21	0	k__Fungi;p__Ascomycota
OTU235	0	0	17	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales
OTU351	0	0	7	0	0	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Ambisporaceae;g__Ambispora;s__unidentified
OTU249	0	0	15	0	4	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU379	0	0	0	17	1	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales
OTU397	0	0	0	3	9	4	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Capnodiales;f__Mycosphaerellaceae;g__Ramularia
OTU650	0	0	0	1	4	12	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales
OTU255	0	0	0	14	0	1	k__Fungi;p__Ascomycota
OTU431	0	0	0	0	0	14	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Cantharellales;f__Ceratobasidiaceae
OTU217	0	15	0	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU265	0	0	0	0	20	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Onygenales;f__Onygenaceae;g__unidentified;s__unidentified
OTU245	0	0	18	0	0	0	k__Fungi;p__Ascomycota;c__Geoglossomycetes;o__Geoglossomycetes_ord_Incertae_sedis;f__Geoglossomycetes_fam_Incertae_sedis;g__Nothomitra;s__Nothomitra_cinnamomea
OTU348	0	0	11	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Tremellodendropsidales;f__unidentified;g__unidentified;s__unidentified
OTU309	0	0	16	0	0	0	k__Fungi
OTU685	0	0	0	0	1	19	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiales_fam_Incertae_sedis;g__Leptodontidium;s__Leptodontidium_trabinellum
OTU450	38	2	0	1	0	2	k__Fungi
OTU628	0	0	0	14	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Fenestellaceae;g__Fenestella;s__Fenestella_mackenziei
OTU415	0	0	6	1	12	0	k__Fungi;p__Ascomycota
OTU549	0	0	13	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Boletaceae;g__Strobilomyces;s__Strobilomyces_strobilaceus
OTU344	0	0	18	0	0	0	k__Fungi;p__Basidiomycota
OTU320	0	0	17	0	0	0	k__Fungi;p__Mucoromycota;c__Mucoromycetes;o__Mucorales;f__Mucoraceae;g__Mucor;s__Mucor_silvaticus
OTU226	0	0	24	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae
OTU520	0	5	11	0	0	3	k__Fungi;p__Ascomycota
OTU190	0	0	16	0	0	0	k__Fungi
OTU304	0	0	12	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Atheliales;f__Atheliaceae;g__Byssocorticium;s__unidentified
OTU542	0	0	15	0	0	0	k__Fungi;p__Ascomycota
OTU324	0	0	14	0	0	0	k__Fungi;p__Glomeromycota
OTU298	24	7	1	4	0	1	k__Fungi;p__Basidiomycota;c__Microbotryomycetes;o__Sporidiobolales;f__Sporidiobolaceae
OTU398	0	0	0	0	11	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Melanogastraceae
OTU21	22	6	0	0	2	0	k__Fungi
OTU430	0	0	0	0	0	17	k__Fungi;p__Ascomycota;c__Sordariomycetes
OTU437	0	0	0	0	0	18	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__Umbelopsidales;f__Umbelopsidaceae;g__Umbelopsis
OTU540	0	0	21	0	1	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium;s__unidentified
OTU261	1	1	1	0	7	7	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__Umbelopsidales;f__Umbelopsidaceae;g__Umbelopsis;s__Umbelopsis_ovata
OTU602	0	0	9	0	0	0	k__Fungi;p__Ascomycota
OTU505	0	17	0	0	0	0	k__Fungi;p__Ascomycota
OTU552	0	0	11	1	0	1	k__Fungi;p__Ascomycota;c__Orbiliomycetes;o__GS33;f__unidentified;g__unidentified;s__unidentified
OTU669	44	0	0	0	1	0	k__Fungi
OTU323	0	0	15	0	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Inocybaceae;g__Inocybe
OTU517	2	1	0	2	5	4	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Chaetomiaceae;g__Chaetomium
OTU684	0	0	0	0	12	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae;g__Hydnobolites;s__unidentified
OTU547	0	0	17	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales
OTU359	0	0	16	0	0	0	k__Fungi
OTU570	0	0	3	0	5	2	k__Fungi;p__Basidiomycota;c__Microbotryomycetes;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU613	0	0	18	0	0	0	k__Fungi
OTU268	0	0	0	1	1	7	k__Fungi;p__Ascomycota;c__Geoglossomycetes;o__Geoglossales;f__Geoglossaceae;g__Trichoglossum
OTU429	0	0	0	0	3	13	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae;g__Archaeospora;s__unidentified
OTU366	0	0	0	16	0	0	k__Fungi
OTU280	8	0	14	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Cyphellaceae;g__Rectipilus;s__Rectipilus_idahoensis
OTU621	0	0	0	8	5	0	k__Fungi
OTU353	0	0	2	1	8	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_changbaiensis
OTU424	0	0	0	0	3	12	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__unidentified;g__unidentified;s__unidentified
OTU219	0	10	0	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales
OTU572	0	0	1	4	0	5	k__Fungi
OTU328	0	0	14	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Xylariales;f__Sporocadaceae;g__Pestalotiopsis;s__unidentified
OTU575	0	0	14	0	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Trechisporales;f__Hydnodontaceae;g__Trechispora
OTU270	0	0	0	0	0	18	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Tremellales;f__Tremellaceae;g__Tremella;s__unidentified
OTU236	15	0	11	0	1	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Dermateaceae;g__Rhizodermea;s__Rhizodermea_veluwensis
OTU456	9	3	0	2	3	1	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Capnodiales
OTU338	0	0	12	0	0	0	k__Fungi
OTU435	0	0	0	0	0	15	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales
OTU559	0	0	13	0	1	0	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae
OTU573	0	0	8	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita;s__Amanita_nivalis
OTU408	0	1	0	0	13	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Polyporales;f__Ganodermataceae;g__Ganoderma;s__Ganoderma_lucidum
OTU342	0	0	14	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Camarophyllopsis;s__Camarophyllopsis_atrovelutina
OTU731	0	0	0	0	0	15	k__Fungi;p__Ascomycota;c__Saccharomycetes;o__Saccharomycetales;f__Phaffomycetaceae;g__Cyberlindnera;s__Cyberlindnera_maclurae
OTU383	0	0	0	10	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Coniochaetales;f__Coniochaetaceae;g__Coniochaeta
OTU561	0	0	12	0	0	0	k__Fungi;p__Ascomycota
OTU678	0	0	0	1	15	0	k__Fungi;p__Ascomycota
OTU422	0	7	0	0	7	0	k__Fungi;p__Basidiomycota;c__Malasseziomycetes;o__Malasseziales;f__Malasseziaceae;g__Malassezia;s__Malassezia_globosa
OTU358	0	0	17	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU284	35	3	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Tricholomataceae;g__Mycena;s__Mycena_abramsii
OTU699	0	1	2	2	1	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Trichocomaceae;g__Talaromyces
OTU407	0	0	0	0	13	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Boletaceae
OTU574	0	0	10	0	0	1	k__Fungi;p__Ascomycota
OTU310	0	0	7	0	0	0	k__Fungi
OTU426	0	0	0	0	2	10	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales
OTU672	0	0	0	0	15	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Bionectriaceae;g__Clonostachys
OTU732	0	0	1	0	0	7	k__Fungi;p__Ascomycota
OTU531	0	0	15	0	0	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pezizaceae
OTU533	0	0	13	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU391	0	0	0	1	15	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU494	0	9	0	0	0	0	k__Fungi;p__Ascomycota
OTU466	4	0	9	0	1	1	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU418	0	0	0	0	14	0	k__Fungi;p__Ascomycota
OTU710	0	0	0	5	5	0	k__Fungi
OTU433	0	0	0	0	0	5	k__Fungi;p__Glomeromycota;c__Glomeromycetes
OTU524	0	0	11	0	0	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Discinaceae;g__Hydnotrya;s__unidentified
OTU378	0	0	0	11	0	0	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae;g__Rhizophagus
OTU671	0	0	0	1	8	0	k__Fungi;p__Basidiomycota;c__Geminibasidiomycetes;o__Geminibasidiales;f__Geminibasidiaceae;g__Geminibasidium;s__unidentified
OTU434	0	0	0	0	0	11	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreales_fam_Incertae_sedis;g__Acremonium
OTU331	0	0	12	0	0	0	k__Fungi
OTU652	23	0	0	3	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Tremellales;f__Bulleribasidiaceae;g__Dioszegia;s__Dioszegia_hungarica
OTU639	0	0	0	5	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_turficola
OTU536	0	0	7	0	0	0	k__Fungi;p__Ascomycota
OTU334	0	0	12	0	0	0	k__Fungi
OTU346	0	0	10	0	0	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes
OTU548	0	0	10	0	0	0	k__Fungi
OTU521	0	2	1	2	7	2	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Onygenales;f__Gymnoascaceae;g__Leucothecium;s__Leucothecium_emdenii
OTU440	24	2	1	0	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Tremellales;f__Bulleribasidiaceae;g__Dioszegia;s__Dioszegia_fristingensis
OTU580	0	1	3	0	2	1	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU316	0	0	13	0	0	0	k__Fungi;p__Mucoromycota;c__Endogonomycetes;o__GS20;f__unidentified;g__unidentified;s__unidentified
OTU254	0	0	0	8	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__unidentified
OTU546	0	0	3	0	0	3	k__Fungi
OTU593	0	0	8	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita
OTU605	0	1	2	0	7	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU635	4	0	0	7	0	4	k__Fungi;p__Ascomycota
OTU557	0	0	13	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU345	3	1	7	0	0	0	k__Fungi;p__Ascomycota
OTU665	0	0	0	0	7	1	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__unidentified;g__unidentified;s__unidentified
OTU411	0	0	0	0	13	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Sclerodermataceae;g__Scleroderma;s__Scleroderma_areolatum
OTU682	0	0	0	0	3	5	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales
OTU632	0	0	0	11	1	1	k__Fungi;p__Ascomycota
OTU641	0	0	0	5	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Cantharellales;f__Cantharellales_fam_Incertae_sedis;g__Sistotrema;s__Sistotrema_sernanderi
OTU395	0	0	0	0	8	0	k__Fungi
OTU350	0	0	12	0	0	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Ambisporaceae;g__Ambispora;s__unidentified
OTU349	0	0	7	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU402	0	0	0	0	6	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Albatrellaceae;g__Leucogaster
OTU34	14	5	0	0	0	0	k__Fungi
OTU327	0	0	9	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae
OTU375	0	0	0	0	9	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__unidentified
OTU692	0	0	0	0	3	3	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU690	0	0	0	0	9	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU341	0	0	6	0	1	3	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_parvispora
OTU376	0	0	0	8	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria
OTU743	0	0	0	0	0	7	k__Fungi
OTU195	0	0	0	5	0	0	k__Fungi;p__Ascomycota
OTU410	0	0	0	0	15	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Hydnangiaceae;g__Laccaria
OTU259	0	0	0	0	8	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU733	0	0	0	0	0	8	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae
OTU677	0	0	0	0	9	0	k__Fungi
OTU312	0	0	6	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Russula;s__Russula_flavida
OTU485	4	9	1	0	0	0	k__Fungi
OTU539	0	0	6	0	1	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae
OTU438	5	0	0	0	0	4	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__unidentified;g__unidentified;s__unidentified
OTU578	1	0	8	0	0	0	k__Fungi;p__Ascomycota
OTU739	0	0	0	0	0	7	k__Fungi;p__Ascomycota
OTU329	0	0	8	0	0	0	k__Fungi;p__Ascomycota;c__Lecanoromycetes
OTU566	0	0	11	0	0	0	k__Fungi;p__Ascomycota
OTU726	0	0	0	0	0	8	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_hyalina
OTU694	0	0	0	0	13	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Nectriaceae
OTU713	0	1	0	0	4	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae
OTU392	0	0	0	0	11	0	k__Fungi
OTU666	0	0	0	0	5	0	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__GS23;f__unidentified;g__unidentified;s__unidentified
OTU560	0	0	11	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Russulales;f__Russulaceae;g__Lactarius;s__Lactarius_deceptivus
OTU37	0	0	3	5	2	0	k__Fungi
OTU550	0	0	7	1	0	0	k__Fungi
OTU352	0	0	7	0	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales
OTU493	0	9	0	0	0	0	k__Fungi
OTU748	0	0	0	0	0	7	k__Fungi;p__Ascomycota
OTU630	0	5	0	0	3	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Cantharellales;f__Clavulinaceae;g__Clavulina;s__Clavulina_cinerea
OTU596	0	0	7	0	0	0	k__Fungi;p__Ascomycota
OTU388	0	0	0	10	0	0	k__Fungi;p__Glomeromycota
OTU670	0	0	5	0	5	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU373	0	0	0	2	6	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales;f__Boletinellaceae;g__Boletinellus;s__Boletinellus_merulioides
OTU629	0	0	1	3	2	1	k__Fungi;p__Basidiomycota;c__Cystobasidiomycetes
OTU409	0	0	0	0	4	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales
OTU489	0	6	1	0	0	0	k__Fungi;p__Ascomycota
OTU367	0	0	0	7	0	0	k__Fungi;p__Ascomycota
OTU662	0	0	1	1	0	2	k__Fungi;p__Mucoromycota;c__Endogonomycetes;o__GS22;f__unidentified;g__unidentified;s__unidentified
OTU527	0	0	7	0	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Trichosporonales;f__Trichosporonaceae
OTU381	0	0	0	5	0	0	k__Fungi;p__Ascomycota
OTU562	0	0	12	0	0	0	k__Fungi;p__Ascomycota
OTU538	0	0	6	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Entolomataceae;g__Entoloma
OTU588	0	0	9	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiaceae;g__Ascocoryne;s__Ascocoryne_sarcoides
OTU38	11	4	0	0	0	0	k__Fungi
OTU428	1	0	0	2	3	4	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae;g__Metarhizium
OTU585	0	0	3	0	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes
OTU87	0	2	2	2	4	0	k__Fungi
OTU544	0	0	8	0	1	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales
OTU534	0	0	4	0	0	0	k__Plantae;p__Anthophyta;c__Eudicotyledonae;o__Fagales;f__Fagaceae;g__Quercus;s__unidentified
OTU599	0	0	5	0	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae
OTU371	0	0	0	5	0	0	k__Fungi;p__Ascomycota
OTU292	0	4	0	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales
OTU606	0	0	6	0	0	0	k__Fungi
OTU609	0	0	1	0	4	1	k__Fungi;p__Chytridiomycota;c__Rhizophydiomycetes;o__Rhizophydiales
OTU427	0	0	0	1	0	7	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU537	0	0	6	0	0	0	k__Fungi;p__Ascomycota
OTU600	0	0	7	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria
OTU723	0	0	0	0	0	7	k__Fungi;p__Ascomycota
OTU535	0	0	6	0	0	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pyronemataceae;g__Scutellinia;s__Scutellinia_cejpii
OTU389	0	0	0	1	0	5	Unclassified
OTU722	0	0	0	0	0	9	k__Fungi
OTU742	0	0	0	0	0	7	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__Umbelopsidales;f__Umbelopsidaceae;g__Umbelopsis;s__unidentified
OTU482	3	1	0	0	0	5	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU416	0	0	0	0	8	0	k__Fungi;p__Ascomycota
OTU619	0	0	6	0	1	0	k__Fungi;p__Ascomycota
OTU519	4	4	0	0	2	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU419	0	0	0	0	9	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__unidentified;g__unidentified;s__unidentified
OTU603	0	0	8	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita
OTU712	0	0	0	0	7	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales
OTU553	0	0	9	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes
OTU468	11	1	0	0	0	1	k__Fungi
OTU399	0	0	0	0	9	0	k__Fungi;p__Basidiomycota;c__Tritirachiomycetes;o__Tritirachiales;f__Tritirachiaceae;g__Paratritirachium;s__unidentified
OTU455	10	2	0	0	0	1	k__Fungi
OTU232	0	0	5	0	0	1	Unclassified
OTU281	7	0	3	0	0	0	k__Fungi;p__Mucoromycota
OTU444	8	0	3	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Sebacinales;f__Sebacinaceae;g__Sebacina;s__unidentified
OTU594	0	0	3	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae
OTU608	0	0	5	0	0	0	k__Fungi;p__Ascomycota
OTU356	0	0	7	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Leotiaceae;g__Leotia;s__Leotia_lubrica
OTU384	0	0	1	3	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU55	3	3	1	1	1	0	k__Fungi
OTU565	0	0	5	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavulinopsis;s__Clavulinopsis_luteoalba
OTU581	0	0	4	0	0	0	k__Fungi;p__Ascomycota;c__Lecanoromycetes;o__Lecanorales
OTU343	0	0	2	0	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Cortinariaceae;g__Cortinarius;s__Cortinarius_hinnuleus
OTU447	10	3	0	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma
OTU620	0	0	2	0	0	0	k__Fungi;p__Ascomycota
OTU702	0	0	0	0	5	0	k__Fungi
OTU673	0	0	0	0	6	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Inocybaceae;g__Inocybe;s__Inocybe_mutata
OTU610	0	0	2	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU443	20	0	0	0	0	0	k__Fungi
OTU532	0	0	5	0	0	0	k__Fungi
OTU488	0	7	0	0	0	0	k__Fungi
OTU724	0	0	0	0	0	5	k__Plantae;p__unidentified;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU302	0	3	0	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiaceae;g__Ascocoryne
OTU50	9	0	0	0	0	0	k__Fungi
OTU618	0	0	8	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Entolomataceae;g__Entoloma;s__Entoloma_alboumbonatum
OTU156	0	5	0	0	0	0	k__Fungi
OTU741	0	0	0	2	0	5	k__Fungi;p__Ascomycota
OTU750	0	0	0	1	1	6	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella
OTU584	0	0	4	0	0	0	k__Fungi;p__Ascomycota
OTU590	0	0	4	0	0	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae;g__Archaeospora;s__unidentified
OTU674	0	0	0	0	6	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Lycoperdaceae;g__Lycoperdon
OTU556	0	0	4	0	0	0	k__Fungi
OTU420	0	0	0	0	6	0	k__Fungi;p__Ascomycota
OTU700	0	0	0	2	5	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Camarophyllopsis;s__Camarophyllopsis_schulzeri
OTU363	0	0	0	5	0	0	k__Fungi;p__Glomeromycota
OTU617	0	0	4	0	0	0	k__Fungi
OTU65	5	1	0	0	0	0	k__Fungi
OTU554	0	0	2	0	0	0	k__Fungi;p__Chytridiomycota;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU683	0	0	0	0	3	0	k__Fungi;p__Basidiomycota;c__Malasseziomycetes;o__Malasseziales;f__Malasseziaceae;g__Malassezia;s__Malassezia_restricta
OTU525	0	0	2	0	0	0	k__Fungi;p__Ascomycota
OTU598	0	0	4	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Leotiaceae;g__Neobulgaria;s__Neobulgaria_pura
OTU504	0	4	0	0	0	0	k__Fungi
OTU463	3	0	5	0	0	0	k__Fungi;p__Ascomycota
OTU506	0	5	2	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Coniochaetales;f__Coniochaetaceae;g__Coniochaeta
OTU278	16	0	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita
OTU479	3	0	0	0	1	0	k__Fungi
OTU439	16	0	0	0	0	0	k__Fungi
OTU625	0	0	0	6	0	0	k__Fungi;p__GS19;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU729	0	0	0	0	0	5	k__Fungi;p__Ascomycota
OTU745	0	0	0	0	0	2	k__Fungi;p__Mucoromycota;c__Mucoromycetes;o__Mucorales;f__Mucoraceae;g__Zygorhynchus;s__unidentified
OTU508	0	5	0	0	0	0	k__Fungi;p__Ascomycota
OTU612	0	0	2	0	0	0	k__Fungi
OTU717	0	0	0	0	4	0	k__Fungi
OTU653	0	0	0	1	4	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae
OTU642	0	0	0	4	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales
OTU737	0	0	0	1	0	1	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales
OTU513	0	3	2	0	0	0	k__Fungi;p__Ascomycota
OTU518	0	1	2	3	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Stachybotryaceae;g__Myrothecium;s__Myrothecium_inundatum
OTU210	6	1	0	0	0	0	k__Fungi
OTU680	0	0	0	0	3	0	k__Fungi;p__Ascomycota;c__Leotiomycetes
OTU499	2	0	0	0	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Capnodiales;f__Mycosphaerellaceae
OTU86	4	2	0	0	0	0	k__Fungi
OTU707	0	0	0	0	5	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Lasiosphaeriaceae;g__Cercophora;s__Cercophora_sparsa
OTU614	0	0	4	0	1	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_globulifera
OTU365	0	0	0	3	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Trichosporonales;f__Trichosporonaceae
OTU459	10	0	1	0	0	0	k__Fungi;p__Ascomycota
OTU75	4	0	0	0	0	0	k__Fungi
OTU491	5	4	0	0	0	0	k__Plantae;p__unidentified;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU648	0	0	0	6	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Xylariales
OTU564	0	0	4	0	0	0	k__Fungi
OTU462	12	0	0	0	1	0	k__Fungi;p__Ascomycota;c__Saccharomycetes;o__Saccharomycetales;f__Saccharomycetales_fam_Incertae_sedis;g__Candida
OTU705	0	0	0	0	1	0	k__Fungi;p__Ascomycota
OTU696	0	0	0	0	5	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Cantharellales;f__Cantharellales_fam_Incertae_sedis;g__Sistotrema
OTU257	0	0	0	2	0	0	k__Fungi
OTU545	0	0	3	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiaceae
OTU734	0	0	2	0	0	2	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreales_fam_Incertae_sedis;g__Acremonium;s__Acremonium_spinosum
OTU476	12	0	0	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Helotiales_fam_Incertae_sedis;g__Cadophora;s__Cadophora_finlandica
OTU708	0	0	0	0	5	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae;g__Pochonia;s__Pochonia_bulbillosa
OTU551	0	0	4	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Ophiocordycipitaceae;g__Tolypocladium;s__unidentified
OTU664	0	0	0	0	2	0	k__Fungi;p__Ascomycota;c__Geoglossomycetes;o__Geoglossales;f__Geoglossaceae;g__Geoglossum;s__Geoglossum_difforme
OTU627	0	0	0	1	0	0	k__Fungi
OTU563	1	0	2	0	1	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Coniochaetales;f__Coniochaetaceae;g__Coniochaeta
OTU651	0	0	1	3	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales
OTU213	4	2	0	0	0	0	k__Fungi
OTU647	0	0	0	1	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Entolomataceae;g__Entoloma;s__Entoloma_terreum
OTU725	1	0	0	0	0	2	k__Fungi;p__Ascomycota
OTU643	0	0	0	4	0	0	k__Fungi;p__Ascomycota
OTU640	0	0	3	1	0	0	k__Fungi;p__GS19;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU706	0	0	0	0	1	2	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae;g__Archaeospora;s__unidentified
OTU529	0	0	4	0	0	0	k__Fungi;p__Ascomycota
OTU644	0	0	0	1	1	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Xylariales;f__Xylariaceae;g__Nemania;s__Nemania_serpens
OTU592	0	0	2	0	0	0	k__Fungi;p__Ascomycota
OTU595	0	0	5	1	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Dermateaceae;g__Mollisia;s__unidentified
OTU661	0	0	0	1	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Entolomataceae;g__Entoloma;s__Entoloma_terreum
OTU744	0	0	0	0	0	4	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Trechisporales;f__Hydnodontaceae;g__Trechispora
OTU401	0	0	0	0	3	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Leotiaceae;g__Leotia;s__Leotia_lubrica
OTU735	0	0	0	0	0	1	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae;g__Archaeospora;s__unidentified
OTU718	0	0	0	0	4	0	k__Fungi;p__Ascomycota
OTU285	3	1	0	1	1	1	k__Fungi
OTU478	4	0	0	2	0	0	k__Fungi
OTU709	0	0	0	0	2	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Inocybaceae;g__Inocybe;s__Inocybe_hystrix
OTU663	1	0	0	1	1	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Clavicipitaceae
OTU589	0	0	4	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes;o__Helotiales;f__Hyaloscyphaceae;g__Arachnopeziza
OTU597	0	0	1	0	0	0	k__Fungi;p__Glomeromycota;c__Archaeosporomycetes;o__Archaeosporales;f__Archaeosporaceae;g__Archaeospora;s__unidentified
OTU569	3	0	0	0	0	0	k__Fungi
OTU583	0	0	3	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae
OTU406	0	0	0	1	1	0	k__Fungi
OTU526	0	0	4	0	0	0	k__Fungi;p__Basidiomycota;c__Microbotryomycetes;o__Leucosporidiales;f__unidentified;g__unidentified;s__unidentified
OTU649	0	0	0	3	0	0	k__Fungi;p__Mucoromycota;c__Umbelopsidomycetes;o__Umbelopsidales;f__Umbelopsidaceae;g__Umbelopsis;s__unidentified
OTU622	0	0	0	2	0	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes
OTU667	0	0	0	0	3	0	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Claroideoglomeraceae;g__Claroideoglomus
OTU287	2	0	0	1	1	0	k__Fungi
OTU260	0	0	0	0	0	1	k__Fungi
OTU117	5	0	0	0	0	0	k__Fungi
OTU541	0	0	2	0	0	0	k__Fungi;p__Glomeromycota;c__Glomeromycetes;o__Glomerales;f__Glomeraceae;g__unidentified;s__unidentified
OTU689	0	0	0	0	2	0	Unclassified
OTU522	0	1	0	2	0	1	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae;g__Penicillium
OTU646	0	0	0	0	1	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Boletales
OTU486	7	1	0	0	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Tremellales;f__Bulleraceae;g__Bullera;s__Bullera_unica
OTU568	0	0	2	0	0	0	k__Fungi;p__Ascomycota
OTU698	0	0	0	0	1	0	k__Fungi;p__Ascomycota
OTU449	9	0	0	0	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetes_ord_Incertae_sedis;f__Gloniaceae;g__Cenococcum
OTU740	0	0	2	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Ophiocordycipitaceae;g__Haptocillium;s__Haptocillium_sinense
OTU477	8	0	0	0	0	0	k__Fungi;p__Ascomycota
OTU465	7	0	0	0	0	0	k__Fungi
OTU160	0	0	0	0	0	1	k__Fungi
OTU657	0	0	0	1	0	1	k__Fungi;p__Ascomycota
OTU631	0	0	0	1	0	0	k__Fungi;p__Ascomycota
OTU130	3	3	0	0	0	0	k__Fungi
OTU582	0	0	2	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Cortinariaceae
OTU715	0	0	0	0	5	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Tricholomataceae;g__Tricholoma;s__Tricholoma_fulvum
OTU567	0	2	1	0	0	0	k__Fungi;p__Ascomycota
OTU688	0	0	0	0	1	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Tremellales;f__Trimorphomycetaceae;g__Saitozyma;s__Saitozyma_podzolica
OTU502	1	2	0	0	0	0	k__Fungi
OTU730	0	0	0	0	0	2	k__Fungi
OTU655	0	0	0	1	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Hypocreales;f__Hypocreaceae;g__Trichoderma;s__Trichoderma_sinuosum
OTU471	5	1	0	0	0	0	k__Fungi
OTU116	4	0	0	0	0	0	k__Fungi
OTU728	0	0	0	0	0	2	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Polyporales;f__Coriolaceae;g__Rigidoporus;s__Rigidoporus_pouzarii
OTU691	0	0	0	0	1	0	k__Fungi;p__Ascomycota
OTU202	0	0	0	0	0	1	k__Fungi
OTU469	4	0	0	0	0	0	k__Fungi;p__Mucoromycota;c__Mucoromycetes;o__Mucorales;f__Rhizopodaceae;g__Rhizopus;s__Rhizopus_arrhizus
OTU687	0	0	0	1	3	0	k__Fungi
OTU458	3	0	1	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Thelephorales;f__Thelephoraceae
OTU421	0	0	0	0	1	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pyronemataceae
OTU738	0	0	0	0	0	2	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Amanitaceae;g__Amanita
OTU369	0	0	0	2	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Atheliales;f__Atheliaceae;g__Athelia
OTU676	0	0	0	0	2	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae;g__Clavaria
OTU364	0	0	0	2	0	0	k__Fungi;p__Chytridiomycota;c__Rhizophydiomycetes;o__Rhizophydiales;f__Rhizophydiaceae;g__Rhizophydium;s__unidentified
OTU637	0	0	0	2	0	1	k__Fungi
OTU711	0	0	0	0	2	0	k__Fungi;p__Ascomycota
OTU484	2	0	0	0	0	1	k__Fungi;p__Ascomycota;c__Saccharomycetes;o__Saccharomycetales;f__Pichiaceae;g__Pichia;s__Pichia_kluyveri
OTU660	0	0	0	3	0	0	k__Fungi;p__Ascomycota
OTU601	0	0	2	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU695	0	0	0	0	1	0	k__Fungi;p__Basidiomycota
OTU326	0	0	3	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes
OTU615	0	0	1	0	0	0	k__Fungi
OTU511	2	0	0	0	0	0	k__Fungi
OTU686	0	0	1	0	1	0	k__Fungi;p__Ascomycota
OTU497	0	2	0	0	0	0	k__Fungi
OTU633	0	1	0	0	0	0	k__Fungi
OTU624	0	0	0	1	0	1	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Chaetosphaeriales;f__Chaetosphaeriaceae
OTU645	0	0	0	1	0	0	k__Fungi
OTU483	3	2	0	0	0	0	k__Fungi
OTU386	0	0	0	2	0	0	k__Fungi
OTU457	5	1	0	0	0	0	k__Fungi
OTU214	3	0	0	0	0	0	k__Fungi
OTU472	3	0	0	0	0	0	k__Fungi
OTU277	3	1	0	0	0	0	k__Fungi
OTU693	0	0	0	0	1	0	k__Plantae;p__unidentified;c__unidentified;o__unidentified;f__unidentified;g__unidentified;s__unidentified
OTU425	0	0	0	0	1	0	k__Fungi;p__Ascomycota;c__Eurotiomycetes;o__Eurotiales;f__Aspergillaceae
OTU297	0	2	0	0	0	0	k__Fungi
OTU611	0	0	1	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Clavariaceae
OTU212	2	0	0	0	0	1	k__Fungi
OTU448	5	0	0	0	0	0	k__Fungi;p__Basidiomycota;c__Tremellomycetes;o__Filobasidiales;f__Piskurozymaceae;g__Solicoccozyma;s__Solicoccozyma_terrea
OTU512	1	1	0	0	1	0	k__Fungi
OTU586	0	0	3	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Trechisporales;f__Hydnodontaceae;g__Brevicellicium;s__Brevicellicium_exile
OTU496	0	2	0	0	0	0	k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Dothideomycetidae_ord_Incertae_sedis;f__Eremomycetaceae;g__Arthrographis;s__unidentified
OTU703	0	0	0	0	3	0	k__Fungi;p__Chytridiomycota;c__Chytridiomycetes;o__Chytridiales;f__Chytriomycetaceae
OTU451	2	2	0	0	0	0	k__Fungi
OTU654	0	0	0	1	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Glomerellales;f__Plectosphaerellaceae;g__Plectosphaerella
OTU208	2	1	0	0	1	0	k__Fungi
OTU498	0	1	0	0	0	0	k__Fungi
OTU288	3	0	0	0	0	0	k__Fungi
OTU507	0	3	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Polyporales;f__Coriolaceae
OTU523	1	2	0	0	0	0	k__Fungi
OTU679	0	0	0	0	3	0	k__Fungi;p__Ascomycota
OTU296	0	1	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes
OTU291	0	2	0	0	0	0	k__Fungi
OTU286	2	0	0	0	0	0	k__Fungi
OTU656	0	0	0	1	0	0	k__Fungi;p__Ascomycota;c__Pezizomycetes;o__Pezizales;f__Pyronemataceae;g__Sphaerosporella;s__unidentified
OTU714	0	0	0	0	2	0	k__Fungi;p__Ascomycota;c__Sordariomycetes
OTU467	3	0	0	0	0	0	k__Fungi
OTU659	0	0	0	3	0	0	k__Fungi
OTU283	3	0	0	0	0	0	k__Fungi
OTU571	0	0	1	0	0	0	k__Fungi;p__Basidiomycota
OTU480	4	0	0	0	0	0	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Atheliales;f__Atheliaceae;g__Piloderma;s__unidentified
OTU747	0	0	0	0	0	1	k__Fungi;p__Basidiomycota;c__Agaricomycetes;o__Agaricales;f__Entolomataceae;g__Entoloma;s__unidentified
OTU279	4	0	0	0	0	0	k__Fungi
OTU470	3	0	0	0	0	0	k__Fungi
OTU746	0	0	0	0	0	2	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Chaetosphaeriales;f__Chaetosphaeriaceae;g__Chaetosphaeria
OTU501	0	1	0	0	0	0	k__Fungi
OTU500	0	1	0	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes
OTU473	2	0	0	0	0	0	k__Fungi
OTU481	3	0	0	0	0	0	k__Fungi
OTU460	3	0	0	0	0	0	k__Fungi
OTU453	3	0	0	0	0	0	k__Fungi
OTU475	2	0	0	0	0	0	k__Fungi
OTU716	0	0	0	0	2	0	k__Fungi;p__Ascomycota
OTU616	0	0	2	0	0	0	k__Fungi;p__Ascomycota;c__Leotiomycetes
OTU490	0	1	0	0	0	0	k__Fungi
OTU503	0	1	0	0	0	0	k__Fungi
OTU579	0	0	1	0	0	1	k__Fungi
OTU461	3	0	0	0	0	0	k__Fungi
OTU675	0	0	0	0	2	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Diaporthales;f__Valsaceae
OTU452	3	0	0	0	0	0	k__Fungi;p__Ascomycota;c__Sordariomycetes;o__Sordariales;f__Cephalothecaceae;g__Phialemonium
OTU510	0	2	0	0	0	0	k__Fungi
OTU668	0	0	0	0	1	0	k__Fungi
OTU638	0	0	0	2	0	0	k__Fungi
OTU474	2	0	0	0	0	0	k__Fungi
OTU515	0	1	0	0	0	0	k__Fungi
OTU454	3	0	0	0	0	0	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_pseudozygospora
OTU720	0	0	0	0	0	1	k__Fungi;p__Mortierellomycota;c__Mortierellomycetes;o__Mortierellales;f__Mortierellaceae;g__Mortierella;s__Mortierella_echinula

3.3.2. OTU heatmap

The heatmap analysis shows the abundance information of selected OTU as well as the similarity and difference across OTUs and samples by similarity clustering. The figure below shows the top 30 OTUs with the highest abundance:

Figure 3.3.2 OTU abundance clustering heatmap

Note: The row name is the OTU ID, the column name is the sample information, the left side of the figure is the OTU cluster tree, and the top is the sample cluster tree. The value of each colored box is the relative abundance of each OTU after normalization.

3.3.3. OTU Venn diagram and petal diagram

According to the results of OTU cluster analysis, the common and unique OTUs of different samples/groups are analyzed. When the number of samples/groups is less than 5, the Venn diagram is drawn. When the sample/group is greater than 5, the petal diagram is drawn.

Figure 3.3.3 OTU Venn diagram or petal diagram

Note: the circles of different colors in the Venn diagram represent different samples or groups, and the numbers in the figure represent the numbers of OTUs unique or common to each sample or group. In the petal diagram, each petal represents a sample or group. The numbers on the petals represent the number of OTUs unique to the sample, and the white circle in the middle represents the number of OTUs shared by all samples and groups.

3.3.4. Species annotation statistics

In order to obtain the classification information of OTU, a representative sequence was selected for each OTU and annotated using the RDP classifier, thereby to obtain the community composition of each sample.

Analysis software: Qiime(1.9.1)

Analysis method: RDP classifier Bayesian algorithm was used to classify the OTU representative sequences of 97% similarity level, and the community composition of each sample was analyzed and summarized at all levels. The comparison database was Greengenes database (http://qiime.org/home_static/dataFiles.html) / 18S rRNA database / ITS database.

For each sample, the percentage of each species at different taxonomic levels (Phylum, Class, Order, Families, Genus, Species) is shown in the table below:

Taxon	C1	C2-2	C3	P1	P2	P3
Unclassified	4,722	6,550	17,283	7,959	8,925	7,270
Mucor	18,074.00	10,057.00	196.00	329.00	231.00	1.00
Russula	368.00	552.00	2,438.00	1,046.00	2,873.00	10,531.00
Sebacina	17.00	0.00	147.00	3,934.00	2,582.00	3,704.00
Pseudotricholoma	45.00	9,258.00	161.00	1.00	2.00	0.00
Saitozyma	214.00	82.00	532.00	3,803.00	2,936.00	1,550.00
Leohumicola	781.00	456.00	328.00	450.00	1,876.00	1,865.00
Elaphomyces	3,600.00	2.00	4.00	2,095.00	3.00	2.00
Geminibasidium	63.00	108.00	1,592.00	3,086.00	478.00	25.00
Sagenomella	26.00	0.00	100.00	173.00	3,509.00	674.00
Lactarius	0.00	0.00	443.00	2,710.00	99.00	297.00
Scleroderma	0.00	0.00	17.00	7.00	2,465.00	3.00
Mortierella	18.00	80.00	1,004.00	255.00	299.00	308.00
Astraeus	0.00	0.00	4.00	1,097.00	346.00	130.00
Penicillium	29.00	56.00	365.00	149.00	300.00	268.00
Trichoglossum	4.00	940.00	0.00	1.00	1.00	11.00
Cenococcum	192.00	44.00	452.00	7.00	198.00	7.00
Clavulinopsis	3.00	0.00	689.00	2.00	6.00	2.00
Trichoderma	11.00	17.00	306.00	166.00	89.00	30.00
Inocybe	0.00	0.00	15.00	310.00	252.00	2.00
Pachyphloeus	0.00	0.00	1.00	2.00	293.00	236.00
Clavaria	1.00	199.00	272.00	16.00	4.00	32.00
Hydnobolites	0.00	0.00	405.00	0.00	14.00	0.00
Tuber	0.00	7.00	344.00	1.00	1.00	0.00
Tylopilus	0.00	0.00	0.00	172.00	0.00	171.00
Amanita	18.00	0.00	144.00	1.00	2.00	171.00
Umbelopsis	8.00	18.00	57.00	17.00	95.00	134.00
Ilyonectria	3.00	3.00	112.00	115.00	88.00	8.00
Chaetosphaeria	0.00	0.00	34.00	2.00	118.00	168.00
Bondarzewia	9.00	3.00	13.00	155.00	135.00	5.00
Trichocladium	25.00	0.00	32.00	56.00	30.00	105.00
Cuphophyllus	0.00	120.00	125.00	0.00	1.00	0.00
Metarhizium	1.00	25.00	35.00	17.00	73.00	89.00
Tomentella	0.00	32.00	9.00	99.00	0.00	99.00
Tricholoma	213.00	0.00	0.00	0.00	5.00	0.00
Pseudogymnoascus	2.00	7.00	21.00	36.00	38.00	104.00
Byssocorticium	0.00	0.00	202.00	0.00	3.00	0.00
Rhizophagus	1.00	0.00	13.00	11.00	0.00	174.00
Gliocladiopsis	0.00	0.00	0.00	1.00	0.00	148.00
Cudoniella	0.00	0.00	0.00	138.00	0.00	0.00
Solicoccozyma	6.00	1.00	52.00	27.00	8.00	29.00
Dendrosporium	9.00	9.00	19.00	38.00	3.00	39.00
Calostoma	0.00	0.00	115.00	1.00	0.00	0.00
Lactifluus	0.00	0.00	0.00	1.00	1.00	101.00
Pseudoclathrosphaerina	0.00	0.00	0.00	40.00	53.00	5.00
Leptodontidium	2.00	5.00	32.00	12.00	5.00	42.00
Pseudotomentella	0.00	0.00	64.00	0.00	2.00	0.00
Chaetomium	2.00	1.00	0.00	29.00	17.00	16.00
Cortinarius	1.00	0.00	61.00	0.00	1.00	1.00
Camarophyllopsis	0.00	0.00	47.00	2.00	5.00	0.00
Neoascochyta	46.00	2.00	0.00	3.00	1.00	2.00
Dioszegia	47.00	2.00	1.00	3.00	0.00	0.00
Entoloma	0.00	0.00	44.00	2.00	0.00	2.00
Genea	0.00	0.00	45.00	0.00	0.00	0.00
Archaeospora	0.00	0.00	5.00	0.00	9.00	29.00
Mycena	35.00	3.00	0.00	0.00	0.00	0.00
Clonostachys	0.00	0.00	0.00	2.00	26.00	10.00
Lycoperdon	0.00	0.00	26.00	0.00	6.00	5.00
Ceratobasidium	0.00	0.00	0.00	0.00	31.00	0.00
Delicatula	3.00	0.00	3.00	0.00	23.00	0.00
Rhizodermea	15.00	0.00	11.00	0.00	1.00	0.00
Dissophora	0.00	0.00	25.00	0.00	0.00	0.00
Asterophora	0.00	0.00	24.00	0.00	0.00	0.00
Nectria	0.00	0.00	0.00	23.00	0.00	0.00
Rectipilus	8.00	0.00	14.00	0.00	0.00	0.00
Coniochaeta	1.00	5.00	4.00	10.00	1.00	0.00
Ramariopsis	0.00	0.00	19.00	0.00	0.00	0.00
Trechispora	0.00	0.00	14.00	0.00	0.00	5.00
Ambispora	0.00	0.00	19.00	0.00	0.00	0.00
Nothomitra	0.00	0.00	18.00	0.00	0.00	0.00
Tremella	0.00	0.00	0.00	0.00	0.00	18.00
Volutella	0.00	0.00	2.00	16.00	0.00	0.00
Malassezia	0.00	7.00	0.00	0.00	10.00	0.00
Ramularia	0.00	0.00	0.00	3.00	9.00	4.00
Cyberlindnera	0.00	0.00	0.00	0.00	0.00	15.00
Gliophorus	0.00	0.00	15.00	0.00	0.00	0.00
Laccaria	0.00	0.00	0.00	0.00	15.00	0.00
Acremonium	0.00	0.00	2.00	0.00	0.00	13.00
Leucothecium	0.00	2.00	1.00	2.00	7.00	2.00
Fenestella	0.00	0.00	0.00	14.00	0.00	0.00
Ganoderma	0.00	1.00	0.00	0.00	13.00	0.00
Pestalotiopsis	0.00	0.00	14.00	0.00	0.00	0.00
Strobilomyces	0.00	0.00	13.00	0.00	0.00	0.00
Candida	12.00	0.00	0.00	0.00	1.00	0.00
Cadophora	12.00	0.00	0.00	0.00	0.00	0.00
Ascocoryne	0.00	3.00	9.00	0.00	0.00	0.00
Hydnotrya	0.00	0.00	11.00	0.00	0.00	0.00
Leotia	0.00	0.00	7.00	0.00	3.00	0.00
Sistotrema	0.00	0.00	0.00	5.00	5.00	0.00
Paratritirachium	0.00	0.00	0.00	0.00	9.00	0.00
Boletinellus	0.00	0.00	0.00	2.00	6.00	0.00
Bullera	7.00	1.00	0.00	0.00	0.00	0.00
Clavulina	0.00	5.00	0.00	0.00	3.00	0.00
Scutellinia	0.00	0.00	6.00	0.00	0.00	0.00
Talaromyces	0.00	1.00	2.00	2.00	1.00	0.00
Myrothecium	0.00	1.00	2.00	3.00	0.00	0.00
Mollisia	0.00	0.00	5.00	1.00	0.00	0.00
Leucogaster	0.00	0.00	0.00	0.00	6.00	0.00
Cercophora	0.00	0.00	0.00	0.00	5.00	0.00
Pochonia	0.00	0.00	0.00	0.00	5.00	0.00
Quercus	0.00	0.00	4.00	0.00	0.00	0.00
Piloderma	4.00	0.00	0.00	0.00	0.00	0.00
Arachnopeziza	0.00	0.00	4.00	0.00	0.00	0.00
Tolypocladium	0.00	0.00	4.00	0.00	0.00	0.00
Neobulgaria	0.00	0.00	4.00	0.00	0.00	0.00
Rhizopus	4.00	0.00	0.00	0.00	0.00	0.00
Brevicellicium	0.00	0.00	3.00	0.00	0.00	0.00
Phialemonium	3.00	0.00	0.00	0.00	0.00	0.00
Pichia	2.00	0.00	0.00	0.00	0.00	1.00
Claroideoglomus	0.00	0.00	0.00	0.00	3.00	0.00
Nemania	0.00	0.00	0.00	1.00	1.00	0.00
Geoglossum	0.00	0.00	0.00	0.00	2.00	0.00
Rhizophydium	0.00	0.00	0.00	2.00	0.00	0.00
Arthrographis	0.00	2.00	0.00	0.00	0.00	0.00
Rigidoporus	0.00	0.00	0.00	0.00	0.00	2.00
Athelia	0.00	0.00	0.00	2.00	0.00	0.00
Zygorhynchus	0.00	0.00	0.00	0.00	0.00	2.00
Haptocillium	0.00	0.00	2.00	0.00	0.00	0.00
Plectosphaerella	0.00	0.00	0.00	1.00	0.00	0.00
Sphaerosporella	0.00	0.00	0.00	1.00	0.00	0.00

Table 3.3.4.1 Taxa Statistics at Genus level

Samples	C1	C2-2	C3	P1	P2	P3
Kingdom	2	2	3	3	3	3
Phylum	8	9	9	9	8	9
Class	18	20	26	22	26	24
Order	30	31	44	42	42	38
Family	40	40	60	56	63	50
Genus	45	39	76	64	70	54
Species	37	36	69	57	65	52

Table 3.3.4.2 Statistics of Taxonomic Composition

3.3.5. Graphics display of species relative abundance

The distribution of the top 30 most abundant classifications in each sample or group at different taxonomic levels (Phylum, Class, Order, Families, Genus, Species) are shown as follows:

Figure 3.3.5.1 Stacked bar plot of species distribution

Note: X axis is the sample name or group name, and the Y axis is the relative abundance of different species. The legend is the name of the taxonomic classification of the species. 'Other' represents the relative abundance of all phylum level classifications other than the top 30.

The top 30 species distribution of each sample (or group) on different levels (Phylum, Class, Order, Families, Genus, Species) was clustered and plotted in a heatmap. The similarity and difference of each species is visualized by color scheme in the heat map. The heat maps plotted for the distribution of the species in each sample on different levels of classification are shown below:

Figure 3.3.5.2 Species distribution heatmap

Note: The columns represent samples and/or groups and the rows represent species. The dendrogram above the heatmap is the cluster result of the samples and/or groups and the dendrogram to the left is the species cluster. The colors in the heat map represent the relative abundance of the corresponding species in the corresponding sample or group.

3.4. Sample complexity analysis

3.4.1. Alpha diversity analysis

In community ecology, alpha diversity is mainly used to reflect the diversity of each sample, which estimates the number of species in the microbial community as well as the abundance and diversity of species in environmental communities through a series of statistical indices.

The indices for community richness calculation include:

ACE: An index to estimate the number OTU in communities. It was proposed by the Chao and is commonly used to estimate the total number of species in ecology. ( http://www.mothur.org/wiki/Ace )

Chao: It is an index that uses the Chao 1 algorithm to estimate the OTU number of samples. Chao is commonly used in ecology to assess the total number of microorganisms.( http://www.mothur.org/wiki/Chao )

The indices for community diversity calculation include:

Shannon: Commonly used to reflect the diversity index a for the estimation of microbial diversity. ( http://www.mothur.org/wiki/Shannon )

Simpson: Simpson diversity index, proposed by Edward Hugh Simpson in 1949 and is commonly used in ecology to quantify biological diversity in a region. ( http://www.mothur.org/wiki/Simpson )

The indices to measure sequencing depth (Coverage) include:

Goods Coverage: Refers to library coverage of each sample. The higher the value, the lower the probability that the sample did not cover the sequence.(http://www.mothur.org/wiki/Coverage )

Analysis software:Qiime (1.9.1)

Analysis method: sequences were randomly extracted and the valid sequences were subject to OTU analysis and a diversity index was calculated for each sample.

Alpha diversity results are summarized in the table below:

sample	ace	chao1	shannon	simpson	goods_coverage
C1	215.44	214	2.46	0.59	1.00
C2-2	218.14	217.73	3.08	0.77	1.00
C3	407.14	406.83	6.34	0.97	1.00
P1	343.04	332.17	4.83	0.93	1.00
P2	416.81	412.77	5.02	0.95	1.00
P3	323.86	344.75	4.80	0.90	1.00

Table 3.4.1 Collation of alpha diversity results

3.4.2. Between-group differential analysis using alpha-diversity indices

To perform between-group a diversity analysis, box plots were generated based on a diversity indices using R, which intuitively displays the maximum, minimum, median and outliers of the a diversity indices of samples in each group as well as the differences between groups.

Analysis method: Box plot was generated using R based on a-diversity index.

The box plot of the inter-group differential analysis based on chao1 and shannon indices is as below:

Note: The left panel is the Chao1 index boxplot of each group. X axis indicates the names of the groups and Y axis indicates the Chao 1 index. Each box diagram shows the minimum, first quartile, medium, third quartile and maximum values of the chao1 index of the corresponding sample. The right graph is the Shannon index boxplot of each group.

3.4.3. Rank-abundance curve

Rank-abundance curve is used to analyze diversity. To generate a rank-abundance curve, the number of valid sequences in each OTU of a given sample was first calculated, and then all the OTUs were ranked in descending order based on their relative abundance (number of valid sequences), and finally the result was plotted with OTU ranking on the X axis and the number of sequences in the OTU on the Y axis. Y axis could also be OTU relative abundance in percentage.

Rank-abundance curve reflects both species abundance and species uniformity. The abundance of species is reflected by the length of the curve on the X axis. The more extended on the X axis, the more abundant the species is. Species uniformity is reflected by the shape of the curve. The smoother the curve, the higher the species uniformity.

Analysis method: R packages were used for graph generation based on the results of OTU analysis

Figure 3.4.3 Rank-Abundance curve

Note: Each curve in the figure above corresponds to an individual sample. The X axis is the relative abundance of the OTU in descending order. The Y axis is the relative abundance of the OTU. '100' on the X axis indicates the OTU in the sample is ranked as the 100th abundant in descending order, and the corresponding value on the Y axis is the percentage of the sequence count in the OTU (the number of sequences of the OTU divided by the total number of sequences).

3.4.4. Rarefaction curve

The rarefaction curve is a useful tool to characterize the species composition of a sample and predicting the abundance of species in a sample. It efficiently deals with the increase of detected species due to the increase in sample size. It is widely used in biodiversity and community surveys to determine whether the sample size is sufficient and to estimate the species abundance. Therefore, the rarefaction curve can not only determine whether the sample size is sufficient, but also predict the species abundance when the sample size is sufficient.

Analysis software: Qiime (1.9.1)

Analysis method: The rarefaction curve was constructed by random sampling. The observed numbers of OTUs were plotted against the number of extracted sequences.

Figure 3.4.4 Observed OTUs rarefaction curves

Note: The X axis is the number of valid sequences extracted, and the Y axis is the number of OTUs (Observed OTUs). Each sample is represented by one curve with a unique color. The number of OTUs increases with the increase of extracted sequence count until reaching a plateau, which indicates the number of detected OTUs will not increase with the amount of extracted sequences and reflects the reasonable sequence depth.

3.5. Multi-sample comparative analysis

3.5.1. (Un)weighted unifrac distance matrix

Beta diversity reflects the diversity and the degrees of differences among samples. The distance between the samples can be calculated using the evolution and abundance information between the sample sequences to reflect whether there is significant difference in microbial community among the samples. This can be achieved by UniFrac analysis.

Analysis software: Qiime (1.9.1)

Analysis method: a phylogenetic tree was constructed using the OTU representative sequences from different environmental samples. The Unifrac metric was then used to measure the difference between two different environmental samples according to the length of the constructed evolutionary tree.

UniFrac analysis includes weighted unifrac and unweighted unifrac methods. The difference between the two is whether to include the relative abundance of sequences from different environmental samples. The weighted unifrac algorithm weights the sequence abundance information when calculating the length of the branch, so unweighted unifrac detects the change among the samples, and the weighted unifrac further quantifies the variation on different pedigrees.

Figure 3.5.1 (Un)weighted unifrac distance matrix heatmap

Note: (Un)weighted unifrac distance matrix heat map. The color scheme in the heatmap represents the degree of difference between the two samples. The lighter the color, the smaller the coefficient between the two samples, and the smaller the difference of species diversity.

3.5.2. PCoA analysis

PCoA (Principal Co-ordinates Analysis) analysis characterizes and visualizes the similarities and differences of data. Similar to PCA, it sorts data based on a series of eigenvalues and eigenvectors and uses the top ranked eigenvalues to determine the most important coordinates in the distance matrix. It also uses eigenanalysis to perform a rigid rotation of the original axes that only changes the coordinates but not the positional relationship of different sample points. The difference between the two is PCA determines the principal components based on sample similarity coefficient matrix whereas PCoA uses distance matrix to find the primary coordinates.

Analysis software: R

Analysis method: Analysis method: PCoA analysis was performed and plotted based on Brary-Curtis distance matrix

Figure 3.5.2 PCoA Plot

Note: Samples of the same group are represented in the same color and shape. PC1_vs_PC2 is the PCoA plot obtained for the first and second principal coordinates; the X and Y axes represent the first and second principal coordinates, respectively. The value in percentage in the axis label represents the contribution of the corresponding coordinate to the sample variance and measures how much this principal is extracted from the original information. The distance between the sample points indicates the similarity of the microbial community in the sample. The closer the points, the higher the similarity. Samples clustered together are composed of similar microbial compositions.

3.5.3. PCA analysis

PCA analysis (Principal Component Analysis) is a statistical technique for the determination of the key variables in a multidimensional data set that are most responsible for the differences in the observations, and thus is commonly used to simplify complex data analysis.

The difference and distance between samples can be reflected by the analysis of the gene functional distribution of different samples. The differences between multiple sets of data can be plotted on a two-dimensional chart using variance decomposition, with the axes representing two eigenvalues that reflects the largest variance values.

Figure 3.5.3 PCA graph

Note: PC1, PC2, PC3 represent the first, second and third principal components, respectively. The percentage after the principal component represents the contribution rate of this component to sample difference and measures how much information the principal component can extract from the original data. The distance between samples indicates the similarity of the distribution of functional classifications in the sample. The closer the distance, the higher the similarity.

3.5.4. NMDS analysis

The non-metric multidimensional scaling is a data analysis method that reduces multi-dimensional space to low-dimensional space to simplify the localization, analysis and classification of research objects. This method preserves the primitive relation among the objects. Its main feature is to position each object in multi-dimensional space based on its functional classification information and calculate the distances between different objects (points) as a measurement of their difference, which are used to obtain the spatial position map.

Analysis method: Graph was generated using vegan package in R based on the beta diversity distance matrix.

Figure 3.5.4 NMDS plot

Note: Each point represents a sample, and the distance between the points indicates the degree of difference. Samples of the same group are represented by the same color. Stress < 0.2 indicates NMDS can accurately reflect the difference between the samples.

3.5.5. UPGMA Tree

Cluster analysis uses evolutionary information derived from sample sequences to calculate whether samples in a specific environment is significantly different from a evolutionary lineage in microbial communities.

Analysis software: Qiime (1.9.1)

Analysis method:The UPGMA (Unweighted pair group method with arithmetic mean) clustering method was used to cluster the samples based on the Brary-Curtis distance matrices.

Figure 3.5.5 UPGMA tree

Note: Each branch in the figure represents a sample. Different colors representing different groups.

3.6. Differential analysis of community composition

3.6.1. Anosim analysis

ANOSIM is a nonparametric test used to examine whether the difference between two or more groups is statistically greater than the within-group difference. It was used to determine whether the grouping is meaningful. R value was obtained by analyzing the sample distance matrix.

Analysis software: vegan package in R

Factor	R-Value	P-Value
Group	0.78	0.10

Table 3.6.1 Group difference evaluation by Anosim

Column description:

(1) Factor:The factors used for grouping

(2) R value: The value of R ranges from 0 to 1. The closer it is to 0, the less significant the between-group difference is compared to within-group difference; the closer it is to 1, the more significance the between-group difference compared to within-group difference.

(3) P value: P-value indicates the statistical credibility, P < 0.05 indicates the result is statistically significant.

The results of the Anosim analysis were ranked by the value of the distances between the two samples. For each group pair, three set of data can be obtained: between-group distances and within-group distances of each of the two groups. The result is shown below in box plot.

Figure 3.6.1 Group comparison by Anosim analysis

Note: X axis is the ranking of the distances of samples. 'Between' represents the result between the two groups, the other two are the results of within-group distance calculation. R values close to 1 indicates the difference between the groups is significantly greater than the within-group difference. P < 0.05 indicates the result is statistically significant.

4. Deliverables

00_Data: Raw data statistics

01_OTU_Taxa: OTU and Taxonomy results

02_Alpha_Diversity: Sample conplexity analysis results

03_Beta_Diversity: Multi-sample comparative analysis results

04_Diff_Comparison: Differential analysis results of community composition

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